Composite

Part:BBa_K3782030

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-20)


Trans-activating crRNA

This part contains a promoter of tracrRNA and the tracrRNA sequence, which is an RNA sequence, much like the crRNA. All together, they form the sgRNA. It can be used together with the Cas9 protein, sgRNA sequence, and donor sequence as a part of the CRISPR/Cas9 technology. This technology is derived from the adaptive immune system of many prokaryotes. Once produced, the Cas9 protein will be associated with a single-guide RNA (sgRNA), which is complementary to the DNA sequence we aim to target and cut. If the sgRNA finds its complementary sequence, Cas9 will induce a double-strand break in proximity of it, opening the DNA double helix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None